Abstract: Objective: To investigate the clinicopathological characteristics of 86 patients with breast cancer and the relationship between nuclear factor-related long noncoding RNA (NKILA) and the distribution of traditional Chinese medicine constitution. Methods: (1) A total of 86 patients diagnosed with breast cancer by pathology from March 2016 to January 2017 at a certain hospital were collected, and their clinicopathological data were organized. (2) Quantitative polymerase chain reaction was used to detect NKILA in patients with breast cancer. (3) The enrolled 86 patients with breast cancer had previously completed the "Traditional Chinese Medicine Constitution Classification and Judgment Form" questionnaire, with complete original data. The expression of NKILA was statistically analyzed in relation to the patients' constitution types and clinicopathological characteristics. Results: (1) The main traditional Chinese medicine constitution types of the 86 patients with breast cancer were primarily Qi stagnation type, blood stasis type, phlegm-dampness type, and damp-heat type. (2) The expression level of NKILA in the 86 patients with breast cancer was influenced by certain clinical indicators such as tumor stage and human epidermal growth factor receptor (Her-2), with statistical significance (P<0.05); however, some indicators such as age did not affect the expression of NKILA, with no statistical significance (P>0.05). Conclusion: (1) The traditional Chinese medicine constitution classification of 86 patients with breast cancer does not affect the expression level of nuclear factor-related long noncoding RNA (NKILA). (2) Breast cancer patients with smaller tumors, fewer lymph node metastases, lower histological grades, earlier tumor stages, and negative Her-2 have higher NKILA expression compared to those with larger tumors, more lymph node metastases, higher histological grades, later tumor stages, and positive Her-2.