Objective：To study the mechanism of Yangjing Capsule through LncRNA H19 regulating StAR to promote testosterone synthesis. Methods：Taking MLTC-1 cells culture in vitro as the model, the water extract of Yangjing capsule (0.01~1mg/ml) was added for 24 hours. The concentrations of testosterone and free testosterone in cell supernatant were measured by ELISA. The effects of Yangjing Capsule on the expression of LncRNA H19mRNA, StAR mRNA and StAR protein in MLTC-1 were detected by qRT-PCR and Western blot. Results：Yangjing Capsule can increase the concentration of testosterone and free testosterone, promote the expression of StAR mRNA and protein, and up-regulate the expression of LncRNA H19 mRNA. Conclusion；Yangjing Capsule can regulate StAR through LncRNA H19, and then increase testosterone synthesis.