目的：探讨赞育丹防治帕罗西汀诱导性功能障碍的效果和机制。方法：将50只雄性Wistar大鼠随机分为5组,每组10 只。对照组给予蒸馏水灌胃,模型组给予帕罗西汀悬浮液10 mg/kg灌胃,赞育丹组给与帕罗西汀悬浮液10 mg/kg与相应剂量的赞育丹低、中、高剂量组分别灌胃给药,连续灌胃4周后观察各组大鼠性行为参数。ELISA检测血清睾酮水平以及鸟苷单磷酸(cGMP)水平。NO测定试剂盒测定阴茎一氧化氮(NO)水平。Western blot检测阴茎eNOS、PDE5的含量。结果：与模型组相比,赞育丹各给药组的骑跨潜伏期,插入潜伏期以及射精潜伏期均剂量依赖性的降低,差异有统计学意义(P均＜0.05)。赞育丹剂量依赖性的升高骑跨次数,与模型组比较差异有统计学意义(P均＜0.05)。赞育丹的中、高剂量组显著增加插入次数,与模型组比较差异有统计学意义(P均＜0.05)。赞育丹各给药组的血清睾酮和阴茎NO、 cGMP水平均随着赞育丹剂量的升高而显著增加(P均＜0.05)。模型组的eNOS含量降低,PDE5的含量升高,赞育丹能显著升高eNOS含量,降低PDE5的含量。结论：赞育丹可以通过提高血清睾酮,提高阴茎eNOS、NO、cGMP水平,抑制PDE5来缓解帕罗西汀引起的性功能障碍。
Objective: This study aimed to explore the effects and mechanism of Zan-Yu-Dan (ZYD) on the paroxetine-induced sexual dysfunction in rats. Methods: Total 50 male Wistar rats were divided into five groups, ten rats in each group. The rats in control group were treated with distilled water by intragastric administration; the rats in the model group were treated with 10 mg/kg paroxetine; the rats in the ZYD groups were administered with 10 mg/kg paroxetine and indicated doses of ZYD, respectively. After treatment for 4 weeks, sexual behavior parameters, testosterone levels, NO, cGMP, eNOS and PDE5 levels among all the groups were measured and compared. Results: Compared with model group, the mount latency (ML), intromission latency (IL) and ejaculatory latency (EL) in ZYD groups were significantly decreased with dose-dependent manner (P<0.05). The mount frequency (MF) in ZYD groups were significantly increased along with the dose-dependent manner (P<0.05). The intromission frequency (IF) in high and middle-dose ZYD group was increased significantly (P<0.05). After the treatment, serum testosterone, penile NO and cGMP levels in ZYD groups were increased significantly with the dose-dependent manner (P<0.05). In the model group, the expression level of eNOS was decreased while that of PDE5 was increased, while ZYD significantly increased the expression level of eNOS and decreased PDE5. Conclusion: Treatment with ZYD significantly alleviated paroxetine-induced sexual dysfunction by increasing serum testosterone levels and penile NO, cGMP and eNOS levels, reducing penile PDE5 level.